标题: Transcriptome analysis of the toxic mechanism of nanoplastics on growth, photosynthesis and oxidative stress of microalga Chlorella pyrenoidosa during chronic exposure
作者: Wenfeng Yang, Pan Gao, Guoyi Ma, Jiayi Huang, Yixiao Wu, Liang Wan, Huijun Ding, Weihao Zhang
来源出版物: Environmental Pollution 卷: 284 文献号: 117413 DOI: /10.1016/j.envpol.2021.117413 出版年:May 19 2021
摘要: The toxicity of nanoplastics to aquatic organisms has been widely studied in terms of biochemical indicators. However, there is little discussion about the underlying toxic mechanism of nanoplastics on microalgae. Therefore, the chronic effect of polystyrene (PS) nanoplastics (80 nm) on Chlorella pyrenoidosa was investigated, in terms of responses at the biochemical and molecular/omic level. It was surprising that both inhibitory and promoting effects of nanoplastcis on C. pyrenoidosa were found during chronic exposure. Before 13 days, the maximum growth inhibition rate was 7.55% during 10 mg/L PS nanoplastics treatment at 9 d. However, the inhibitory effect gradually weakened with the prolongation of exposure time. Interestingly, algal growth was promoted for 1–5 mg/L nanoplastics during 15–21 d exposure. Transcriptomic analysis explained that the inhibitory effect of nanoplastics could be attributed to suppressed gene expression of aminoacyl-tRNA synthetase that resulted in the reduced synthesis of related enzymes. The promotion phenomenon may be due to that C. pyrenoidosa defended against nanoplastics stress by promoting cell proliferation, regulating intracellular osmotic pressure, and accelerating the degradation of damaged proteins and organs. This study is conducive to provide theoretical basis for evaluating the actual hazard of nanoplastics to aquatic organisms.
KeyWords: Chronic exposure; Inhibition effect; Microalgae; Nanoplastics; Promotion effect
地址: [Wenfeng Yang, Guoyi Ma, Jiayi Huang, Yixiao Wu, Liang Wan, Weihao Zhang] Wuhan Univ, Sch Resource & Environm Sci, 129 Luoyu Rd, Wuhan 430079, Peoples R China.
[Pan Gao] Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China.
[Huijun Ding] Jiangxi Provincial Key Laboratory of Water Resources and Environment of Poyang Lake, Jiangxi Institute of Water Sciences, Nanchang, Jiangxi 330029, PR China.
通讯作者地址: weihao. zhang (通讯作者)，Wuhan Univ, Sch Resource & Environm Sci, 129 Luoyu Rd, Wuhan 430079, Peoples R China.
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